Recent advances and perspectives of nucleic acid detection for coronavirus
Identifieur interne : 000332 ( 2020/Analysis ); précédent : 000331; suivant : 000333Recent advances and perspectives of nucleic acid detection for coronavirus
Auteurs : Minzhe Shen ; Ying Zhou ; Jiawei Ye ; Abdu Ahmed Abdullah Al-Maskri ; Yu Kang ; Su Zeng ; Sheng CaiSource :
- Journal of Pharmaceutical Analysis [ 2095-1779 ] ; 2020.
Abstract
The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) in Chinais posing a great threat to global public health. Therefore, rapid and a accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving people’s lives and preventing epidemics. It is important to establish a quick standard diagnostic test for the detection of the infectious disease (COVID-19) to prevent subsequent secondary spread. Polymerase chain reaction (PCR) is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity. Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler operation. A variety of improved or new approaches also have been developed. This review summarizes the currently available detection methods for coronavirus nucleic acid. It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coronavirus infection.
Url:
DOI: 10.1016/j.jpha.2020.02.010
PubMed: 32292623
PubMed Central: 7102540
Affiliations:
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PMC:7102540Le document en format XML
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Therefore, rapid and a accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving people’s lives and preventing epidemics. It is important to establish a quick standard diagnostic test for the detection of the infectious disease (COVID-19) to prevent subsequent secondary spread. Polymerase chain reaction (PCR) is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity. Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler operation. A variety of improved or new approaches also have been developed. This review summarizes the currently available detection methods for coronavirus nucleic acid. 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Hardick</name></author><author><name sortKey="Metzgar, D" uniqKey="Metzgar D">D. Metzgar</name></author><author><name sortKey="Risen, L" uniqKey="Risen L">L. Risen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wright, A V" uniqKey="Wright A">A.V. Wright</name></author><author><name sortKey="Nunez, J K" uniqKey="Nunez J">J.K. Nunez</name></author><author><name sortKey="Doudna, J A" uniqKey="Doudna J">J.A. Doudna</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gootenberg, J S" uniqKey="Gootenberg J">J.S. Gootenberg</name></author><author><name sortKey="Abudayyeh, O O" uniqKey="Abudayyeh O">O.O. Abudayyeh</name></author><author><name sortKey="Lee, J W" uniqKey="Lee J">J.W. Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Freije, C A" uniqKey="Freije C">C.A. Freije</name></author><author><name sortKey="Myhrvold, C" uniqKey="Myhrvold C">C. Myhrvold</name></author><author><name sortKey="Boehm, C K" uniqKey="Boehm C">C.K. Boehm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gootenberg, J S" uniqKey="Gootenberg J">J.S. Gootenberg</name></author><author><name sortKey="Abudayyeh, O O" uniqKey="Abudayyeh O">O.O. Abudayyeh</name></author><author><name sortKey="Kellner, M J" uniqKey="Kellner M">M.J. Kellner</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Abdullah Al Maskri, Abdu Ahmed" sort="Abdullah Al Maskri, Abdu Ahmed" uniqKey="Abdullah Al Maskri A" first="Abdu Ahmed" last="Abdullah Al-Maskri">Abdu Ahmed Abdullah Al-Maskri</name><name sortKey="Cai, Sheng" sort="Cai, Sheng" uniqKey="Cai S" first="Sheng" last="Cai">Sheng Cai</name><name sortKey="Kang, Yu" sort="Kang, Yu" uniqKey="Kang Y" first="Yu" last="Kang">Yu Kang</name><name sortKey="Shen, Minzhe" sort="Shen, Minzhe" uniqKey="Shen M" first="Minzhe" last="Shen">Minzhe Shen</name><name sortKey="Ye, Jiawei" sort="Ye, Jiawei" uniqKey="Ye J" first="Jiawei" last="Ye">Jiawei Ye</name><name sortKey="Zeng, Su" sort="Zeng, Su" uniqKey="Zeng S" first="Su" last="Zeng">Su Zeng</name><name sortKey="Zhou, Ying" sort="Zhou, Ying" uniqKey="Zhou Y" first="Ying" last="Zhou">Ying Zhou</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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